Fig. 4.

Gene expression at early stages of regeneration (stage 2/3 and stage 4/5). a Colour coded sagittal scheme of stage 2/3 and frontal schemes of stages 2/3 and 4/5. Crossed arrows near schematic indicate the axis of the section. Schematics of gene expression in b and c follow the sagittal scheme as this view allows clear distinction of all tissues. Pictures of gene expression in whole mount are taken in frontal view from the aboral side after orienting the samples, unless otherwise specified. b Whole mount ISH at two regenerative stages (stage 2/3 and stage 4/5) as indicated at the top of the columns using antisense probes for transcription factors. Probe name is indicated on the left of the summary schematics. c Whole mount ISH at two regenerative stages (stage 2/3 and stage 4/5) as indicated at the top of the columns using antisense probes for known differentiation genes. Probe name is indicated on the left of the summary schematics. In b and c, summary schematics of expression are based on several images of different focal plane observations of multiple samples; however, only one focal plane is shown here. A, aboral; O, oral; R, right; L, left; P, proximal; D, distal. Dark blue/purple indicates probe-specific signal. Scale bars = 100 μm