Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 19;11:21. doi: 10.1186/s13578-021-00531-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 3 — Tumor formation and tissue differentiation by cortical NPCs from E13.5 mouse embryos. a IF staining of neural stemness marker expression in neurospheres formed by cortical NPCs cultured in serum-free medium. Cell nuclei were counter-stained with DAPI. b Xenograft tumors formed by NPCs in nude mice. c Comparison of tumor growth by NE-4C and cortical cells within a same period. Significance of difference in tumor volume between two groups was calculated using two-way ANOVA-Bonferroni/Dunn test. Data are shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. NS: not significant. d An overview and specific types of tissue differentiation in an HE-stained tumor section. Original objective magnifications: 4× for overview, 40× for specific tissues. e, f Differential expression of genes representing mesendodermal tissue differentiation (e), neural stemness and neuronal differentiation (f) in NPCs and xenograft tumors, as detected with RT-qPCR. Significance of expression change was calculated based on experiments in triplicate using two-tailed Student’s t-test. Data are shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. NS: not significant. g IHC detection of cell/tissue markers in tumor sections. Below the marker panels are corresponding sections stained with HE. Objective magnification: 20×; insets: 40×