Fig. 4.

Difference in ability of formation of neurosphere-like structures by different cells in serum-free culture. a Different phenotypes of cancer cell lines when cultured in their normal culture medium and in serum-free medium. NE-4C neural stem cells and MEFs were cultured as controls. b IF detection of the neural stemness marker Sox1/SOX1 in these cells that were cultured in serum-free medium. Nuclei were counterstained with DAPI. c Culture of MSCs in serum-free medium