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. 2021 Jan 19;11:21. doi: 10.1186/s13578-021-00531-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Tissue differentiation in HCT 116 and U-118 MG xenograft tumors. a, b RT-qPCR detection of expression of genes for neuronal and neural stemness (a) and genes for mesodermal or/and endodermal tissue differentiation (b) in HCT 116 cells and tumors. c IHC detection of cell/tissue markers in HCT 116 tumor sections. Below the panels for marker expression are corresponding sections stained with HE. d, e RT-qPCR detection of expression of genes for neuronal and neural stemness (d) and genes for mesodermal or/and endodermal tissue differentiation (e) in U-118 MG cells and tumors. f IHC detection of cell/tissue markers in U-118 MG tumor sections. Below the panels for marker expression are corresponding sections stained with HE. In (a, b) and (d, e), significance of gene expression change was calculated based on experiments in triplicate using two-tailed Student’s t-test. Data are shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. NS: not significant. In c and f, objective magnification for sections: 20×; insets: 40 ×