Extended Data Figure 9: Secondary structure arrangement and sequence alignment.

Secondary structures based on the hsPANX1 structure model are labeled. The W74 forming the extracellular entry is marked with an arrow. Key residues forming the side tunnel are labeled with a red asterisk. The cysteine residues forming the extracellular disulfide bonds are highlighted by an orange dot. The N255 glycosylation site is marked with a green dot. The gap junction interface and caspase 3/7 cleavage site are indicated with a red frame. A gain-of-function disease mutation (Δ21–23) of hsPANX1 is also marked.