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. 2021 Jan 18;12:64. doi: 10.1186/s13287-020-02117-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — miR-204-5p is downregulated during the osteogenic differentiation of hADSCs. a Flow cytometry was used to identify hADSCs by detecting specific marker proteins CD44, CD45, CD90, CD105 and HLA-DR. b Relative expression of miR-204-5p at each time point during the osteogenic differentiation of hADSCs detected by qRT-PCR. U6 was used as a reference gene. Relative mRNA levels of the osteogenic markers RUNX2, ALP and OCN at each time point during the osteogenic differentiation of hADSCs detected by qRT-PCR. GAPDH was used as an internal control gene. Data are presented as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.01 (n = 3 independent experiments)