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. 2021 Jan 18;12:64. doi: 10.1186/s13287-020-02117-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — miR-204-5p overexpression inhibits the expression of RUNX2, ALP and OCN. hADSCs were transfected with miR-204-5p mimics, miR-204-5p inhibitors or NC and cultured in PM or OM. a Relative mRNA levels of miR-204-5p, RUNX2, ALP and OCN measured by qRT-PCR on day 14 of osteogenic induction. U6 and GAPDH were used as internal control genes. The data were normalized to the NC-PM group. b Left panel: Western blot of RUNX2, ALP and OCN protein levels on day 14 after osteogenic induction. GAPDH was used as an internal control. Right panel: quantitative analysis of the protein levels. c Confocal microscopy of cells with OCN with DAPI counterstaining on day 14 after osteogenic induction. Scale bars, 20 μm. Data are presented as the mean ± SD. *hADSCs transfected with miR-204-5p mimics or inhibitors vs NC in PM; ^#hADSCs transfected with miR-204-5p mimics or inhibitors vs NC in OM. * or ^#p < 0.05, ** or ^##p < 0.01, *** or ^###p < 0.001 (n = 3 independent experiments)