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. 2021 Jan 18;12:64. doi: 10.1186/s13287-020-02117-4

Fig. 4.

Fig. 4

FOXC1 reduces the suppressive effect of miR-204-5p on the expression of GDF7, RUNX2, ALP and OCN. hADSCs were co-transfected with miR-204-5p mimics and FOXC1 overexpression vector or empty vector or co-transfected with miR-204-5p inhibitors and si-FOXC1 and cultured in OM. a Relative expression of miR-204-5p, FOXC1, GDF7, RUNX2, ALP and OCN measured by qRT-PCR on day 14 of osteogenic induction. U6 and GAPDH were used as internal control genes. b Left panel: Western blot of FOXC1, GDF7, RUNX2, ALP and OCN protein levels on day 14 after osteogenic induction. GAPDH was used as an internal control. Right panel: quantitative analysis of the protein levels. Data are presented as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 (n = 3 independent experiments)