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. 2021 Jan 18;12:64. doi: 10.1186/s13287-020-02117-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — FOXC1, as a transcription factor, binds to the promoter of miR-204-5p and GDF7. a Predicted binding site of FOXC1 (GGTAAGTA) in the miR-204-5p promoter (− 922 to − 915 bp). b Relative luciferase activity of cells transfected with vector/vector-FOXC1 and miR-204-5p-WT or vector/vector-FOXC1 and miR-204-5p-MUT. Data are presented as the mean ± SD. *p < 0.05 (n = 3 independent experiments). c Predicted binding site of FOXC1 (GCCAAGTA) in the GDF7 promoter (− 700 to − 693 bp). d Relative luciferase activity of cells transfected with vector/vector-FOXC1 and GDF7-WT or vector/vector-FOXC1 and GDF7-MUT. Data are presented as the mean ± SD. ***p < 0.001 (n = 3 independent experiments). e The enrichment of FOXC1 in the promoter of GDF7 (left and right panel) and miR-204-5p (right panel) was measured by gel electrophoresis and ChIP assay. Normal IgG was used as a negative control. f The enrichment of histone deacetylase HDAC2 in the promoter of miR-204-5p was measured by gel electrophoresis and ChIP assay. g The enrichment of histone acetylase H3K9AC in the promoter of miR-204-5p was measured by gel electrophoresis and ChIP assay. Data are presented as the mean ± SD. ***anti-HDAC2 or anti-H3K9AC vs IgG, ^###overexpression of FOXC1 vs vectors in HDAC2 or H3K9AC samples. ***p < 0.001, ^###p < 0.001 (n = 3 independent experiments)