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. Author manuscript; available in PMC: 2021 Aug 12.
Published in final edited form as: Nano Lett. 2020 Jul 15;20(8):6032–6037. doi: 10.1021/acs.nanolett.0c02048

Figure 1.

Figure 1.

Construction of DNA-tethered nanodiscs (DND). (a) DNA conjugation reaction with cholesterol (1), phospholipid (2), protein transmembrane domain (3), or MSP (4). (b) SDS-PAGE stained for DNA (left) or protein (right) of each product, (1)-(4). (c) DND formation (DND1-DND4) by incorporating corresponding DNA-anchor amphiphile ((1)-(4)) in the ND reconstitution mixture. (d) SDS-PAGE and TEM characterization of DND1-DND4. Homogeneous circular particles in TEM images (right) indicate proper ND formation. Protein ladder bands (from top to bottom): 250, 130, 100, 70 (red), 55, 35, 25 (red), 15 kDa. Scale bar: 25 nm.