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[Preprint]. 2021 Jan 15:2021.01.13.424628. [Version 1] doi: 10.1101/2021.01.13.424628

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Fig. 3 — Vortex emulsification with qPCR readout enables accurate bulk ddPCR. (a) The DNA sample is added to a tube, oil with stabilizing surfactant is introduced, and the mixture emulsified by vortexing. (b) Vortexed emulsions are thermally cycled. An aliquot is amplified with TaqMan probes to enable visualization. (c) Size distribution of the vortex emulsified droplets obtained by imaging (n = 1323). (d) qPCR readout of vortex-emulsified ddPCR allows accurate quantitation of targets over a range of concentrations.