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. Author manuscript; available in PMC: 2021 Jan 19.
Published in final edited form as: Neuron. 2019 Oct 2;104(4):749–764.e6. doi: 10.1016/j.neuron.2019.08.029

Figure 6. Zic2 Spinal Neurons Synapse with Spinal Motor Neurons.

Figure 6.

(A) Intersectional strategy to exclusively visualize Zic2creER spinal neurons. CAG promoter; FRT and loxP, recognition sites for Flp and Cre recombinases, respectively.

(B) Transverse section of a P15 lumbar SC Zic2-creER;Cdx2FlpO::Ai65D immunolabeled with mCherry (red) and ChAT antibodies (green). Note tdTomato+ axons surrounding motor neurons (B’).

(C) Experimental strategy to visualize Zic2creER pre-synaptic terminals (green) and retrogradely labeled spinal motor neurons with CTB injections in the quadriceps muscle (red).

(D) Transverse section of Zic2creER; Taulox-STOP-lox-SynGFP lumbar SC retrogradely traced with CTB (red) and immunolabeled with GFP (green) and vGluT2 antibodies (blue). SynGFP marks Zic2+ terminals co-localizing with vGluT2 on CTB labeled motor neurons (D’ and D”).

Scale bars, 100 mm (B and D), 10 mm (B’), 5 mm (D’), and 2 mm (D”).