FIGURE 6.
Relative transcript levels of regulators were measured in Pectobacterium atrosepticum strains wild type (WT) and ∆eda. P. atrosepticum SCRI1039 WT and ∆eda mutant were grown at 28 °C under different culture conditions (minimal medium [MM] plus 0.2% pyruvate and supplemented with or without polygalacturonic acid [PGA]) to OD600 0.4–0.6. The recA gene was used as a control housekeeping gene. Transcript levels of wild type in pyruvate were set as zero and the other three treatments were normalized to the wild type. The expression levels of up‐regulated genes were positive while those of down‐regulated genes were negative (a)–(f). Error bars represent standard errors for triplicate assays. *p < .05, **p < .01, NS, no significant difference, Duncan's multiple range test