Skip to main content
. Author manuscript; available in PMC: 2021 Dec 18.
Published in final edited form as: ACS Chem Biol. 2020 Nov 21;15(12):3099–3105. doi: 10.1021/acschembio.0c00755

Figure 3. In vitro analysis of phosphoribosyltransferase activity of TgUPRT variants with different uracil analogs.

Figure 3.

a. Specific activity of TgUPRT variants with different uracil analogs (1 to 4 as shown in Fig. 2A). n = 3 technical replicates. Error bars are standard deviation of the mean b. Time course analysis of 2 (5VU) analog incorporation into RNA by streptavidin dot blot (left panels) along with immunoblot analysis (right panels) of corresponding 6xHis-TgUPRT protein levels. α-His = anti-His antibody for immunoblot. Strep-HRP = Streptavidin conjugated Horseradish peroxidase was used for assessment of biotin levels resulting from clicked RNA. MeBl = Methylene blue stain and CS = Coomassie staining served as a loading control of RNA and proteins, respectively.