Figure 5.

Mature Eggs Translocate into the Lumen of the Intestines

(A–I) Quantification (A, C, E, and H) and representative brightfield images (B, D, F, G, and I) of mature and immature eggs found in the liver (A and B), small and large intestinal wall tissue and vasculature (C and D), small intestinal luminal content (E–G), and large intestinal luminal content (H and I) for six individual S. mansoni-infected mice.

(B and D) Representative images with image (left) showing immature (yellow arrow) and mature (white arrowhead) magnified from yellow square in wide-field image (right).

(F and G) Images of eggs from the lumen of the small intestine, showing two mature eggs in contact with one immature egg (F), and a wide-field image showing three mature eggs (G).

(I) Representative image of an egg recovered from feces at low resolution (left) and higher resolution with developed miracidia visible (right).

(J) Pooled data for mice 2, 3, 4, and 6 from (A, C, E, and H). SI, small intestine; LI, large intestine.

(K) Dimensions of eggs from this experiment that were classified as immature or mature (open or closed circles, respectively) plotted with eggs shed in the feces of S. mansoni-infected humans (Martinez, 1916). All scale bars are 100 μm except for (G) and the right panels of (B and D), which are 300 μm. ND, not determined. Statistics, Fisher’s exact test. Also see Figure S5.