Figure 5.

Silencing of miR-129-3p inhibits calcium overload and oxidative stress in glucose fluctuation-exposed hippocampal neuronal cells. Rat primary hippocampal neuronal cells were transfected with miR-NC, miR-129-3p mimics, miR-129-3p inhibitor with or without NAC followed by glucose fluctuation treatment. (A–C) The concentration of mitochondrial Ca²⁺ and O₂⁻ was detected using immunofluorescence. Scale bars = 50 μm. Data are presented as the means ± SD; **p< 0.01; (n = 5). CT, normal glucose; GV, glucose fluctuation; antio, NAC.