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. 2021 Jan 15;14:153–163. doi: 10.2147/DMSO.S285179

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© 2021 Wang et al.

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miR-129-3p inhibits ROS-mediated apoptosis in glucose fluctuation-exposed hippocampal neuronal cells. Rat primary hippocampal neuronal cells were transfected with miR-NC, miR-129-3p mimics, miR-129-3p inhibitor with or without NAC, followed by glucose fluctuation treatment. (A) Flow cytometry was performed to detect the apoptosis of hippocampal neuronal cells. (B) Apoptosis rate (%). (C) Mitochondrial cytochrome c and (D) cytosolic cytochrome c. mRNA levels of (E) caspase-9 and (F) caspase-3. Data are presented as the means ± SD; *p< 0.05, **p< 0.01; (n = 5). CT, normal glucose; GV, glucose fluctuation; antio, NAC.