Figure 3.

O-GlcNAc highly expressed in bladder cancers and increased NUSAP1 expression. (A) WB analysis of the expression of NUSAP1 and O-GlcNAc in 25 paired bladder cancer tissues and tissues adjacent to cancer. One representative figure was shown. (n=25, **P<0.01, ***P<0.001, tumor tissues group vs normal tissues). (B) WB was performed to determine the protein expression of NUSAP1 and O-GlcNAc in normal uroepithelium cell line SV-HUC-1 and bladder cancer cell line HT-1376, T24 and 5637. (n=3, ***P<0.001, HT-1376, T24 and 5637 group vs SV-HUC-1 group). (C and D) HT-1376 and T24 cells were treated with PuGNAc (25 mM), GlcNAc (4 mM) or PuGNAc + GlcNAc for 24 h, then cells were collected for WB analysis to detect the expression of O-GlcNAc and NUSAP1 (n=3, *P<0.05,***P<0.001, PuGNAc, GlcNAc or PuGNAc + GlcNAc group vs control group).