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. 2021 Jan 19;16(1):e0245618. doi: 10.1371/journal.pone.0245618

Fig 5. Deletion of Myog impairs transcription assembly at muscle gene promoters.

Fig 5

A-C. ChIP assays were performed on stable C2C12 cells with Myog deletion using antibodies against MYOD1, MYOG, SSRP1, TBP, RNAPII and histone 3(H3), and primers spanning Tnni2(A), Lmod2(B), and Myh3(C) promoters. Rabbit IgG was used as a background control. Standard errors (S.E.) from the mean (Mean ± S.E.) represents the error bars. (Student t.test; ns represents ‘not significant’, *p<0.05, **p<0.01 and ***p<0.001, n = 3–5 biological replicates except C where n = 2 biological replicates).