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Three-dimensional potential of mean force, ΔGPMF, and the projection onto a two-dimensional subspace representing the relative free energy profile based on the calculation of the probability distributions for two atom pair distances, d1 (defined by the distance from G60 Cα atom to GDP/GTP Pβ atom) and d2 (defined by the distance from T35 Cα atom to GDP/GTP Pβ atom) for (A) K-Ras4A-GDP, (B) K-Ras4B-GDP, (C) K-Ras4A-GTP, and (D) K-Ras4B-GTP.
