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. 2021 Jan 19;19:25. doi: 10.1186/s12951-021-00772-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Conjugation of norovirus-like particles. a Silver-stained SDS-PAGE gel of the vaccine components. The noro-VLP appears as a double band between the 70 and 50 kDa markers. All wells contain approximately 250 ng of protein. b Western blot analysis of noro-VLP decorated with the influenza antigens. The HisTag in the N-termini of SpyCatcher fusion proteins is recognized by mouse anti-HisTag antibody. c Dynamic light scattering analysis of SpyTag-noro-VLP and SpyTag-noro-VLP decorated with M2e and HA2. Numerical data shown in Additional file 1: Figure S4. Representative transmission electron microscopy pictures with arithmetic means of equivalent circle diameters ± standard deviation of d native noro-VLP (n = 114), e SpyTag-noro-VLP (n = 112) and noro-VLP decorated with f M2e (n = 109) and g HA2 (n = 274). The scale bars are all 200 nm. 60,000× magnification was used for these pictures and analysis