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. 2020 May 8;92(11):7596–7603. doi: 10.1021/acs.analchem.0c00174

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This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License, which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.

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Protein oxidation for tubulin beta chain. Calculated ln(PF) for tubulin beta chain oxidatively modified peptides identified by (a) label-free MS (N = 11) and (b) cPILOT (N = 6) across three biological replicates. SASA calculated values using the Homo sapiens tubulin beta chain cryo-EM structure (PDB: 5N5N(31)) are displayed on top of each bar. (c) Oxidatively modified peptides identified by label-free MS only (red), cPILOT only (blue), and both methods only (yellow) mapped on the cryo-EM structure of the human tubulin beta chain. CID-MS/MS spectra of the tubulin beta chain peptide 325–336 showing b- and y-ions for (d) light and (e) heavy dimethylation plus a +16 FPOP modification for residue M330 and isobaric-tag. HCD-MS³ spectra generated from the 10 most intense fragment ions (SPS-10) of the (f) light and (g) heavy CID-MS/MS ion.