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. 2021 Jan 6;10:e63726. doi: 10.7554/eLife.63726

Figure 2. Dlg5 and Slmap partially co-localise with Dlg1 during T-tubule morphogenesis.

(A–C) Wild-type developing flight muscles at 32 hr, 48 hr, and 90 hr after puparium formation (APF) co-stained for T-tubule marker Dlg1 with Dlg5 (A) or Slmap (B) or with another T-tubule marker Amphiphysin (C). (D) Wild-type flight muscles at 90 hr APF co-stained for Amphiphysin and actin with phalloidin. Scale bars represent 2 µm.

Figure 2.

Figure 2—figure supplement 1. Dlg5 and Slmap protein knock-down efficiency.

Figure 2—figure supplement 1.

(A, B) Longitudinal sections of all DLMs at 32 after puparium formation (APF) comparing wild type to (A) Dlg5-IR and (B) Slmap-IR muscle fibers, stained for actin and Dlg5 (A) or Slmap (B). Note the decreased levels of antibody labelling in knock-down muscles. Scale bars represent 50 µm.