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. 2021 Jan 6;10:e63726. doi: 10.7554/eLife.63726

Figure 6. The Hippo pathway is essential for post-mitotic muscle fiber growth.

(A, B) Longitudinal sections of all dorsal-longitudinal flight muscles (DLM) at 24 hr, 32 hr, 48 hr, and 90 hr after puparium formation (APF) (A) and digital cross-sections of DLM4 at 24 hr, 32 hr, and 48 hr APF (B) from wild type, Dlg5-IR and yorkie-IR TubGAL80ts Mef2-GAL4 UAS-Diap1 (shifted to 31°C at 0 hr APF) stained for actin. Scale bars represent 50 µm for longitudinal sections and 10 µm for cross-sections. In B dotted lines highlight the fiber areas. (C) Box plots showing DLM4 fiber length, digital cross-sectional area, and volume (calculated by multiplying length with digital cross-sectional area for each pupa) at 24 hr and 48 hr APF. Student’s t test, *** p-value<0.001, * p-value<0.05.

Figure 6.

Figure 6—figure supplement 1. Apoptosis block and muscle growth.

Figure 6—figure supplement 1.

(A) Hemi-thoraces at 90 hr after puparium formation (APF) from Dlg5-IR and yorkie-IR TubGAL80ts Mef2-GAL4 UAS-GFP-Gma pupae, in which GAL4 activity was restricted to pupal stages (shift to 31°C at 0 hr APF). Note the empty thoraces in these double UAS-lines, which serve as controls for GAL4 dilution for Figure 6. (B) Longitudinal sections of flight muscles at 48 hr and 90 hr APF from UAS-p35 and UAS-p35 Dlg5-IR Mef2-GAL4 stained for actin. The dotted lines highlight the cuticle and scale bars represent 100 µm. (C) Digital cross-section of dorsal-longitudinal flight muscle 4 (DLM4) at 48 hr APF from UAS-p35 Dlg5-IR Mef2-GAL4 stained for actin. Dotted lines highlight the fiber area. Note the small fiber cross-section area. Scale bar represent 10 µm.