Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. melanogaster) | w- | BDSC: 3605 | ||
| Genetic reagent (D. melanogaster) | Dlg5-IR-1 | VDRC: 22496 | Pruned from the VDRC collection | |
| Genetic reagent (D. melanogaster) | Dlg5-IR-2 | VDRC: 101596 | ||
| Genetic reagent (D. melanogaster) | Dlg5-IR-3 | BDSC: 30925 | w[*]; P{w[+mC]=UAS-Dlg5.RNAi}3, source: Helena Richardson | |
| Genetic reagent (D. melanogaster) | Slmap-IR-1 | VDRC: 8199 | ||
| Genetic reagent (D. melanogaster) | Slmap-IR-2 | BDSC: 32509 | y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS00513}attP2 | |
| Genetic reagent (D. melanogaster) | Cka-IR-1 | BDSC: 28927 | y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.HM05138}attP2 | |
| Genetic reagent (D. melanogaster) | Cka-IR-2 | VDRC: 35234 | ||
| Genetic reagent (D. melanogaster) | Strip-IR | BDSC: 34657 | y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.HMS01134}attP2 | |
| Genetic reagent (D. melanogaster) | yorkie-IR-1 | VDRC: 111001 | ||
| Genetic reagent (D. melanogaster) | yorkie-IR-2 | VDRC: 40497 | ||
| Genetic reagent (D. melanogaster) | warts-IR-1 | VDRC: 111002 | ||
| Genetic reagent (D. melanogaster) | warts-IR-2 | BDSC: 41899 | y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.GL01331}attP2 | |
| Genetic reagent (D. melanogaster) | Hippo-IR | VDRC: 104169 | ||
| Genetic reagent (D. melanogaster) | sd-IR | VDRC: 101497 | ||
| Genetic reagent (D. melanogaster) | kon-IR | VDRC: 106680 PMID:24631244 |
||
| Genetic reagent (D. melanogaster) | UAS-Dlg5-GFP | BDSC: 30927 | w[*]; P{w[+mC]=UAS-Dlg5.RNAi}2, source: Helena Richardson | |
| Genetic reagent (D. melanogaster) | Mef2-GAL4 | BDSC: 27390 | y[1] w[*]; P{w[+mC]=GAL4-Mef2.R}3 | |
| Genetic reagent (D. melanogaster) | UAS-Flp; Mef2-GAL4 | this study | Mef2GAL4 on third combined with UAS-Flp on X | |
| Genetic reagent (D. melanogaster) | UAS-GFP-Gma | BDSC: 31776 PMID:11784030 |
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| Genetic reagent (D. melanogaster) | Tub-GAL80-ts | BDSC: 7016 PMID:14657498 |
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| Genetic reagent (D. melanogaster) | hippo[wt] | Duojia Pan, UT Soutwestern, USA PMID:29262338 |
tub-FRT-y+-FRT-FLAG-hpo | |
| Genetic reagent (D. melanogaster) | hippo[4A/431T] | Duojia Pan, UT Soutwestern, USA PMID:29262338 |
tub-FRT-y+-FRT-FLAG-hpo[4A/431T] | |
| Genetic reagent (D. melanogaster) | Act88F-GAL4 | Richard Cripps, San Diego State University, USA PMID:22008792 |
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| Genetic reagent (D. melanogaster) | UAS-Hippo | Barry Thompson, John Curtin School of Medical Research, Australia PMID:14502295 |
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| Genetic reagent (D. melanogaster) | UAS-Diap1 | BDSC: 6657; Barry Thompson, John Curtin School of Medical Research, Australia | w[*]; P{w[+mC]=UAS-DIAP1.H}3 | |
| Genetic reagent (D. melanogaster) | UAS-p35 | BDSC:5072; Barry Thompson, John Curtin School of Medical Research, Australia | ||
| Genetic reagent (D. melanogaster) | yorkie-CA | BDSC: 28836; Barry Thompson, John Curtin School of Medical Research, Australia PMID:18256197 |
y[1] w[*]; P{w[+mC]=UAS-yki.S168A.GFP.HA}10-12-1 | |
| Genetic reagent (D. melanogaster) | UAS-Yki | Richard G. Fehon, University of Chicago, USA PMID:30032991 |
wild type Yorkie tagged with FLAG | |
| Genetic reagent (D. melanogaster) | UAS-myr-Yki | Richard G. Fehon, University of Chicago, USA PMID:30032991 |
membrane tethered wild type Yorkie tagged with FLAG | |
| Genetic reagent (D. melanogaster) | Mhc-GFP | VDRC: 318471 PMID:26896675 |
fTRG500 | |
| Genetic reagent (D. melanogaster) | Act88F-GFP | VDRC: 318362 PMID:26896675 |
fTRG10028 | |
| Genetic reagent (D. melanogaster) | Prm-GFP | VDRC: 318114 PMID:26896675 |
fTRG475 | |
| Genetic reagent (D. melanogaster) | Sls-GFP [CA06744] | Allan Spradling, Carnegie Institution for Science, Maryland, USA PMID:17194782 |
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| Chemical compound, drug | GFP-Booster Atto488 | ChromoTek GmbH gba488 |
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| Chemical compound, drug | Rhodamine Phalloidin | Thermo Fischer Scientific R415 |
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| Chemical compound, drug | Vectashield with DAPI | Biozol VEC-H-1200 |
||
| Chemical compound, drug | Fluoroshield with DAPI | Sigma F6057 |
||
| Chemical compound, drug | anti-Dlg5, rabbit | this study | ||
| Chemical compound, drug | anti-Slmap, rabbit | this study | ||
| Chemical compound, drug | anti-Dlg1, mouse | DSHB: 4F3 PMID:11709153 |
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| Chemical compound, drug | anti-Hippo, guinea pig | Georg Halder, VIB-KU Leuven, Belgium PMID:16341207 |
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| Chemical compound, drug | anti-Amphiphysin, rabbit | Jörg Großhans, Georg-August-Universität, Göttingen, Germany PMID:11711432 |
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| Chemical compound, drug | Tissue-Tek O.C.T. | Weckert Labotechnik 4583; Sakura Finetek |
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| Chemical compound, drug | FEATHER Microtome Blades, C35 | pfmmedical, Cologne, Germany 207500003 | ||
| Chemical compound, drug | TRIzol | Thermo Fischer Scientific 15596026 |