Figure 1. Experimental design.

We propagated budding yeast lines in 96-well microplates in one of three environmental conditions, using a daily dilution protocol as shown at top. Each population was founded by a single clone of one of three ancestral genotypes (a haploid MATa, a haploid MATα, and a diploid, all derived from the W303 strain background). On a weekly basis, we froze all populations in glycerol at −80°C for long-term storage. The frozen timepoints used for the analyses in this paper are indicated at bottom.