Fig. 6.

Hedgehog signaling is active but not essential for tubule morphogenesis in vitro. a GLI1 expression (blue line) over the course of 6 days in in vitro tubules. Day 0, n = 7; day 1, n = 5; days 2–6, n = 4. GLI1 expression upon treatment with cyclopamine (red line). n = 3. Bars indicate minimum and maximum RQ. b Tubule formation at 1 and 3 days. Scale bars 500 μm. c Tubule formation at 1 and 3 days upon treatment with cyclopamine. Scale bars 500 μm