Skip to main content
. 2020 Dec;9(6):2411–2427. doi: 10.21037/tlcr-20-1250

Figure 3.

Figure 3

Sirt1 is a target gene of miR-138 regulated by hsa_circ_0006571. (A) The expression of Sirt1 and the EMT markers E-cadherin and Vimentin after transfection with miR-138 mimics in H1975 and SPC-A1 cells. (B) WT and MUT sequences designed for Sirt1 according to the binding site for miR-138. (C) Luciferase reporter assays confirmed the binding of miR-138 with Sirt1. (D) RIP assay was performed using anti-Ago2 in SPC-A1 cells, and the coprecipitated miR-138 was subjected to qRT-PCR for hsa_circ_0006571. (E) RNA pull-down assay confirmed hsa_circ_0006571 binding to miR-138. (F) A high expression of Sirt1 was observed in SM tissues compared with that in lung adenocarcinoma tissues, as determined by Western blotting. (G) Sirt1 expression in SM tissues was analyzed by qRT-PCR (n=20). (H) Expression levels of Sirt1 and Vimentin in SM tissues and adjacent normal tissues were examined using IHC (200×). *, P<0.05; **, P<0.01. circRNAs, circular RNAs; Sirt1, silent information regulator-1; EMT, epithelial-mesenchymal transition; miRNAs, microRNAs.