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. 2020 Dec;9(6):2440–2459. doi: 10.21037/tlcr-20-1272

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2020 Translational Lung Cancer Research. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Effect of GM-CSF on EMT in radiated pulmonary fibrosis. (A) Western blot analysis of EMT markers in radiated lung tissues with/without GM-CSF treatment at 10 weeks. Quantification analysis of raw density of Western blot figures are measured in different groups. (B) GM-CSF reduced the expression levels of ECA, VIM and TGF-β1. Representative images of IHC staining of ECA, VIM, and TGF-β1 are assessed in lung tissues from different groups at 10 weeks. Error bar indicates mean ± standard deviation (n=6; **, P<0.01). GM-CSF, granulocyte-macrophage colony-stimulating factor; EMT, epithelial-mesenchymal transition; ECA, E-cadherin; VIM, vimentin; IHC, immunohistochemistry.