. 2020 Dec;9(6):2440–2459. doi: 10.21037/tlcr-20-1272

Table 2. Primers used for amplification of quantitative real-time polymerase chain reaction (qRT-PCR).

Gene names	Primer names	Primer sequences
GAPDH	Forward	5'-GGTGCCTGTCGTTGTGTTC-3'
GAPDH	Reverse	5'-GCTCCTTCTGGTGCTGTTG-3'
TNF-α	Forward	5'-CATGCGTCCAGCTGACTAAA-3'
TNF-α	Reverse	5'-TCCCCTTCATCTTCCTCCTT-3'
IL-6	Forward	5'-CCACTTCACAAGTCGGAGGCTTA-3'
IL-6	Reverse	5'-TGCAAGTGCATCATCGTTGTTC-3'
IL-1β	Forward	5'-TTTAGCATGCCTGCCCTGAA-3'
IL-1β	Reverse	5'-AGCCCTCCATTCCTGAAAGC-3'
Snail	Forward	5'-ATGGAGTGCCTTTGTACCCG-3'
Snail	Reverse	5'-TGAGGGAGGTAGGGAAGTGG-3'
Vimentin	Forward	5'-AGAGAGGAAGCCGAAAGCAC-3'
Vimentin	Reverse	5'-TGGATCTCTTCATCGTGCAGT-3'
Mmp-9	Forward	5'-CCATGCACTGGGCTTAGATCA-3'
Mmp-9	Reverse	5'-GGCCTTGGGTCAGGCTTAGA-3'
TGF-β1	Forward	5'-TACGGCAGTGGCTGAACCAA-3'
TGF-β1	Reverse	5'-CGGTTCATGTCATGGATGGTG-3'
E-cadherin	Forward	5'-CAGATGATGATACCCGGGACAA-3'
E-cadherin	Reverse	5'-TGCAGCTGGCTCAAATCAAAG-3'
Collagen-1	Forward	5'-GACATGTTCAGCTTTGTGGACCTC-3'
Collagen-1	Reverse	5'-GGGACCCTTAGGCCATTGTGTA-3'
Fibronection-1	Forward	5'-ATCATAGTGGAGGCACTGCAGAA-3'
Fibronection-1	Reverse	5'-GGTCAAAGCATGAGTCATCTGTAGG-3'
Occludin	Forward	5'-AAGTGAATGGCAAGCGATCATA-3'
Occludin	Reverse	5'-CTGTACCGAGGCTGCCTGAA-3'
Sftpc	Forward	5'-GCTACATCATGAAGATGGCTCCAG-3'
Sftpc	Reverse	5'-ACACAGGGTGCTCACAGCAAG-3'