Table 1:
Characteristics of cell cultured in the developed FMi-OOC compared to those seen in conventionally used in vitro models, as well as to the human in vivo conditions (* Red font highlights key differences that do not recapitulate those of human in vivo environment).
| Characteristics | Cell Type | 2D Cell Culture | 3D Cell Culture |
FMi-OOC | Human |
|---|---|---|---|---|---|
| Morphology | AEC | Cuboidal/Fibroblastoid | Fibroblastoid | Cuboidal/Fibroblastoid | Cuboidal/Fibroblastoid |
| AMC | Fibroblastoid | Cuboidal/Fibroblastoid | Fibroblastoid | Fibroblastoid | |
| CMC/CT | Fibroblastoid/Cuboidal | Fibroblastoid/Cuboidal | Fibroblastoid/Cuboidal | Fibroblastoid/Cuboidal | |
| Decidua | Fibroblastoid | Fibroblastoid | Fibroblastoid | Fibroblastoid | |
| Collagen production | AEC | low | high | high | high |
| AMC | high | high | high | high | |
| CMC/CT | low | high | high | high | |
| Decidua | low | low | low | low | |
| Intermediate filament expression | AEC | Metastate | Mesenchymal | Metastate | Metastate |
| AMC | Mesenchymal | Metastate | Mesenchymal | Mesenchymal | |
| CMC/CT | Mesenchymal/Epithelial | Mesenchymal/Epithelial | Mesenchymal/Epithelial | Mesenchymal/Epithelial | |
| Decidua | Mesenchymal | Mesenchymal | Mesenchymal | Mesenchymal |