Skip to main content
. Author manuscript; available in PMC: 2021 Jan 19.
Published in final edited form as: J Am Chem Soc. 2008 Aug 21;130(37):12328–12333. doi: 10.1021/ja8038079

Figure 4.

Figure 4.

Enantiomeric excess calibration curves obtained using a 96-well plate reader. (a,b) Absorbance at 602 nm as a function of ee for displacement experiments performed in a solution containing CAS (10 μM), Cu(OTf)2 (200 μM), and (R,R)-1 (2.5 mM) in 1:1 MeOH:H2O, 50 mM HEPES buffered to pH 7.5, with the addition of (a) aspartate (302 μM) or (b) histidine (202 μM). (c,d) Absorbance at 602 nm as a function of ee for displacement experiments performed with the addition of amino acid into a solution containing CAS (10 μM), Cu(OTf)2 (105 μM), and (R,R)-2 (8.8 mM) in 1:1 MeOH:H2O, 50 mM HEPES buffered to pH 7.5, with the addition of (c) alanine (149 μM) or (d) valine (125 μM).