Fig. 5.
Anti-metastasis efficacy in 4T1-induced metastatic model. (a) The therapeutic regime for BALB/c mice inoculated with 5 × 105 4T1-luciferase cells per mouse via tail vein injection. (b, c) In vivo bioluminescence images of anaesthetized tumor-bearing mice (b) and the average bioluminescence intensity of various groups at different time points (c) (n = 5, data presented as mean ± s.d,one-way ANOVA with Tukey test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). (d) Representative H&E staining images of lung tissues from various groups. (e,f) Analysis of the number (e) and tumor area (f) of lung metastasis nodules after various treatments (n = 5, data presented as mean ± s.d, one-way ANOVA with Tukey test, *P < 0.05, **P < 0.01, ****P < 0.0001). (g) Survival curves for various groups (n = 8, log-rank test, **P < 0.01, ***P < 0.001, ****P < 0.0001).