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. 2021 Jan 6;11:613830. doi: 10.3389/fimmu.2020.613830

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Copyright © 2021 Tsai, Amerman and Jewell

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Polyplexes with different arginine modifications on Trp2 retain ability to deliver immune signals and expand T cells, but Trp2 loading in polyplexes influences T cell functionality. Flow cytometry was used to measure (A) CpG uptake and (B) Trp2R_x uptake following 24h incubation with Trp2R₃, Trp2R₆, or Trp2R₉ polyplexes. Representative histograms for T cells cultured with treated DCs demonstrate similar levels of T cell proliferation across different (C) numbers of arginine modification and (D) Trp2R_x : CpG ratio, with (E) quantification of proliferation by dilution of CFSE MFI. (F) ELISAs of supernatants from DC/T cell co-cultures reveal that IFN-γ secretion levels vary with number of arginine modifications and Trp2R_x : CpG ratio, with higher IFN-γ correlating with formulations with higher Trp2 loading. Different letters indicate statistical significance among means (p <.05).