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. 2021 Jan 6;11:588679. doi: 10.3389/fphys.2020.588679

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Copyright © 2021 Végh, Verkerk, Cócera Ortega, Wang, Geerts, Klerk, Lodder, Nobel, Tijsen, Devalla, Christoffels, Medina-Ramírez, Smits, Tan, Wilders, Goumans and Boink.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Pilot experiments of Hcn2 and SkM1 currents measured at 36°C and room temperature, respectively, in single nucleofected CPCs. (A) Top panel, protocol used. Middle panel, recording of an empty CPC. Bottom panel, Hcn2-encoded “funny current” (at test potentials ranging from –30 to –120 mV) in an Hcn2 nucleofected CPC. (B) Top panel, protocol used. Middle panel, recording of an empty CPC. Bottom panel, skeletal fast sodium current (at test potentials ranging from –50 to +20 mV) in a SkM1 nucleofected CPC. SkM1 was measured from a holding potential of –140 mV.