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. 2021 Jan 6;11:588679. doi: 10.3389/fphys.2020.588679

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Copyright © 2021 Végh, Verkerk, Cócera Ortega, Wang, Geerts, Klerk, Lodder, Nobel, Tijsen, Devalla, Christoffels, Medina-Ramírez, Smits, Tan, Wilders, Goumans and Boink.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GFP expression and CPC engraftment 1 week after transplanting CPCs to the left ventricle near the left anterior descending artery. (A–C) Typical examples of expression of GFP (green), β1-integrin (red), and Collagen-1 (white) in mouse hearts injected with (A) unmodified CPCs, (B) LV-GFP transduced CPCs or (C) GFP nucleofected (NF) CPCs. Inset: GFP expression alone. Scale bars represent 100 μM. (D–F) Expression of GFP, β1-integrin, and Collagen-1 in mice injected with unmodified CPCs (n = 4), lentivirally transduced CPCs (n = 4) or nucleofected CPCs (n = 5), measured as% positive area of total left ventricular area. * indicates P < 0.05.