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. 2021 Jan 19;32(1):1. doi: 10.1007/s10856-020-06475-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Extracellular matrix mineralization. A Von kossa staining on day 7 (n = 12 for each group) and B on day 21 (n = 12 for each group); C quantification of extracellular matrix mineralization by te Von kossa assay on day 21 using Image J software (NIH) (n = 12 for each group). Different lower-case letters refer to a significant difference (ANOVA/Tukey test, p < 0.05) among groups at the same experimental time; D tetracycline fluorescence on day 7 (n = 12 for each group) and E on day 21 (n = 12 for each group); F quantification of mineralization nodules by the tetracycline assay on day 21 (n = 12 for each group). Different lower-case letters refer to a significant difference (ANOVA/Tukey test, p < 0.05) among groups at the same experimental time; G red alizarin quantification on day 21 (n = 12 for each group). Different lower-case letters refer to a significant difference (ANOVA/Tukey test, p < 0.05) among groups at the same experimental time