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. 2020 Nov 8;80(2):250–260. doi: 10.1136/annrheumdis-2020-218493

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© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution 4.0 Unported (CC BY 4.0) license, which permits others to copy, redistribute, remix, transform and build upon this work for any purpose, provided the original work is properly cited, a link to the licence is given, and indication of whether changes were made. See: https://creativecommons.org/licenses/by/4.0/.

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Cell numbers of (A) total leucocytes, (B) neutrophils, (C) macrophages, (D) CD3+ populations, (E) CD4+ populations, (F) CD8+ populations, (G) CD19+ populations and (H) the M1-like/total macrophage ratio determined by flow cytometry in tumour necrosis factor (TNF)-tg and TNF-tg^11βKO animals receiving vehicle or corticosterone (100 µg/mL) in the drinking water for 3 weeks. Gene expression (AU) of (I) Ccl2, (J) Cxcl2, (K) Cxcl10, (L) Il-1, (M) mTnf and (N) Gilz determined by quantitative PCR in tibia isolated from TNF-tg and TNF-tg^11βKO animals receiving vehicle or corticosterone (100 µg/mL) in the drinking water for 3 weeks. Values are expressed as mean±SE, n=6 per group. Statistical significance was determined using two-way analysis of variance with Tukey post hoc analysis. *P<0.05, **p<0.005, ***p<0.001.