Figure 4.

Fusion affected by extension of the length or flexibility of the synaptobrevin-2 JLR. (A) Domain structure of full length synaptobrevin-2 with 3, 7, 9 GSG insertions after K⁸⁵ (K⁸⁵-3i, K⁸⁵-7i, K⁸⁵-9i) in the JLR. (B,C) Lipid mixing (B) and content mixing (C) between synaptobrevin-2 WT, K⁸⁵-3i, K⁸⁵-7i, and K⁸⁵-9i liposomes and syntaxin-1/SNAP-25 liposome. (D,E) Lipid mixing (D) and content mixing (E) between synaptobrevin-2 WT, K⁸⁵-3i, K⁸⁵-7i, and K⁸⁵-9i liposomes and Munc18-1/syntaxin-1 liposome. (F) Domain structure of full-length synaptobrevin-2 with 3, 7, 9 GSG insertions after L⁹³ (L⁹³-3i, L⁹³-7i, L⁹³-9i) in the JLR. (G,H) Lipid mixing (G) and content mixing (H) between synaptobrevin-2 WT, L⁹³-3i, L⁹³-7i, and L⁹³-9i liposomes and syntaxin-1/SNAP-25 liposome. (I,J) Lipid mixing (I) and content mixing (J) between synaptobrevin-2 WT, L⁹³-3i, L⁹³-7i, and L⁹³-9i liposomes and Munc18-1/syntaxin-1 liposome. Representative traces came from one of three independent experiments. Bars on the right panel in (B–E,G–J) are Means ± SDs, n = 3.