Figure 4.

The expression pattern of GLAST/EAAT1 is altered in cerebellum (A,B) and brain (C,D) after CCl₄ treatment. (A) GLAST/EAAT1 immunoreactivity in cerebellum from mice treated with one dose of CCl₄ or three doses of CCl₄. (B) Quantification of GLAST/EAAT1 protein expression signal intensity in cerebellum tissue. (C) GLAST/EAAT1 immunoreactivity in brain from mice treated with one dose of CCl₄ or three doses of CCl₄. (D) Quantification of GLAST/EAAT1 protein expression signal intensity in brain tissue. Results are expressed as mean ± SD (n = 3–5 animals per group). Cerebellum tissue from mouse was used as positive control for the detection of GLAST/EAAT1 expression. An unpaired t-test with Welch's correction was performed to compare between the groups, **p < 0.01, *p < 0.05 vs. vehicle-treated mice (control).