FIGURE 5.

CircRNA expression variation in molecular subtypes of JMML. (A) QRT-PCR expression data of 6 circRNAs in 21 JMML of 5 molecular subtypes [KRAS, NRAS, PTPN11 and NF1 mutated, and quintuple negative (5N)], and 6 age-matched HD samples. CircRNA quantification in not less than 17 patients was obtained for all the circRNAs; differential expression of KRAS, NRAS, and PTPN11 groups compared with HD (Kruskal–Wallis test) and to each other was tested with Mann–Whitney U test; only p-values < 0.1 are shown for the corresponding pairwise comparison. (B) Expression heatmap of circRNAs differentially expressed between JMML molecular subtypes according to RNA-seq data (sample expression is given as row scaled values). The first group of bars on the left of the heatmap show Log₂(Fold Change) comparing KRAS, NRAS, and PTPN11 groups to each other and with HD (“Fold change”); the second group of bars show the significance of differential expression in pairwise JMML subgroups comparisons (“Significance”); “AE,” per row average of absolute expression.