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. 2021 Jan 19;4:89. doi: 10.1038/s42003-020-01576-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a, b Representative histological Hematoxylin & Eosin (H&E), Safranin-O/Fast-green (Saf-O), and Alizarin Red S (ARS) staining of sagittal sections of hMSC tube explants containing TGF-β1-loaded, BMP-2-loaded, or TGF-β1 + BMP-2-loaded microparticles at weeks 3 and 6. c, d Representative H&E staining and in situ hybridization for human Alu repeats of transaxial hMSC tube explant sections at weeks 3 and 6. Scale bars, 100 μm (dotted squares in insets show region of interest in high magnification image).