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. 2021 Jan 19;10(1):12. doi: 10.1038/s41389-020-00300-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A–D Proliferation of sarcoma cells grown as spheroids (A, C) and western blot for Nanog, Bcl-2, cleaved caspase-3, and γ-H2AX (B, D) following treatment with sh.NANOG or sh.Scr and doxorubicin (Dox) or 6 Gy radiotherapy. β-Actin, loading control. *p < 0.01 compared to control. E Immunofluorescence of sarcoma spheroid cells (HT1080, SK-LM-1). Top panel shows γ-H2AX in green. Middle panel shows γ-H2AX in green and nuclei in blue (DAPI). Bottom panel shows Comet assay in green. All photos are of different cells under specified conditions. Graphs show quantification of γ-H2AX positive staining and mean tail moment. Nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole; blue).