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. 2021 Jan 19;10(1):12. doi: 10.1038/s41389-020-00300-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Western blot of HT1080 cells transduced with sh.NANOG lentivirus for Nanog and β-actin. B Growth of HT1080 fibrosarcoma xenografts in athymic nude mice treated with sh.NANOG or sh.Scr and PBS or doxorubicin (Dox). C Photos of representative tumors. D Immunohistochemical analysis of tumors for Ki67 (green), CD133 (green), Nanog (white), cleaved caspase-3 (red), and Bcl-2 (yellow). Scale bar, 50 µm. *p < 0.01 compared to control.