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. 2021 Jan 19;11:1815. doi: 10.1038/s41598-020-80168-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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GMAP mapping result and the comparison of goldfish transcript isoforms between the PacBio Iso-Seq and reference genome annotation. (a) Statistics of the consensus sequences mapped onto the reference genome. (b) Classification of transcript isoforms corresponding to the reference genome. (c) The comparison of gene numbers between Pacbio Iso-Seq and the reference genome annotation. (d) Classifications of novel gene loci identified in PacBio Iso-Seq. (e) The comparison of transcript length distribution between Pacbio Iso-Seq and the reference genome annotation. (f) Comparisons of the average length and N50 length of transcripts between Pacbio Iso-Seq and the reference genome annotation. (g) The comparison of transcript isoform number between Pacbio Iso-Seq and the reference genome annotation. The figures were constructed in ggplot2 (https://ggplot2.tidyverse.org).