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. 2020 Dec 28;40:101851. doi: 10.1016/j.redox.2020.101851

Fig. 4.

Fig. 4

MiR-150 and miR-495 enhance TGFβ1-induced FAO repression in HKC-8 cells. (A, B) Oxygen consumption rate (OCR) of HKC-8 cells transfected with 40 nM miR-NC and mimic miR-150-5p (A) or miR-495-3p (B) and exposed to 10 ng/ml TGF-β1 after miRNA overexpression (see also methods section). Bar graphs (right panels) show the rates of OCR associated to basal, proton-leak, ATP-linked, maximum and reserve capacities and non-mitochondrial respiratory statuses. Data are represented after normalization by protein amount. (C, E) ATP levels in HKC-8 cells transfected with miR-NC and mimic miR-150-5p (C) or miR-495-3p (E). (D, F) mRNA levels of CPT1A, PGC1A and TFAM in HKC-8 cells transfected with miR-NC and mimic miR-150-5p (D) or miR-495-3p (F) were determined by qRT-PCR using Sybr green. Bar graphs show the mean ± s.e.m. of 4 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 compared to their corresponding control conditions; #P < 0.05, ##P < 0.01, ###P < 0.001 compared to cells treated with miR-NC with the same experimental condition.