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. 2021 Jan 19;11:19. doi: 10.1186/s13568-020-01178-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — The phylogenetic tree of the 40 verified xylanase genes and some xylanase genes downloaded from available database based on amino acid identity analyses with MEGA. The genes labeled yellow are xylanases with high activity retrieved from the enzyme repository of the ExPaSy database accessed at https://enzyme.expasy.org/. The microbial gene sources and their accession numbers are: Aspergillus niger XP_001388522.1, Bispora sp. MEY-1 ACS96449.1, Penicillium sp. ACY70400.1, Schizophyllum commune P35809.1, Talaromyces cellulolyticus BAO51921.1, Trichoderma reesei ACB38137.1, Aspergillus sp. Q4WG11.1, Neurospora crassa Q7SDQ1, Streptomyces coelicolor NP_733679.1, Bacillus subtilis P18429, Streptomyces sp. S27 ACF57948.1, Paenibacillus campinasensis Q2I6W5, Paenibacillus curdlanolyticus BAK22544.1, Bacillus sp. ACR47980.1, Arthrobacter sp. AGC01501.1, Streptomyces olivaceoviridis AHK22787.1. The 40 gene sequences with blue and red markings were expressed in E. coli; the ones marked red were expressed with xylanase activity, whereas the ones marked blue were expressed without xylanase activity. The two proteins labeled with two red star pentagons were selected to determine their xylanase properties after purification