Table 1.
The 40 cloned xylanase genes and their closest gene counterparts in the GenBank database
| Name | AAa | Closest genes and their accession numbers | Identity (%) | Sb |
|---|---|---|---|---|
| PW-xyl1 | 333 | TPA: 1,4-β-xylanase [Cyanobacteria bacterium] (UBA11371) (HAZ45611.1) | 55.66 | |
| PW-xyl2 | 360 | Endo-1,4-β-xylanase [uncultured bacterium Contig15] (AHF23866.1) | 77.87 | |
| PW-xyl3 | 368 | Endo-1,4-β-xylanase [Hallella seregens] (WP_052323291.1) | 75.68 | |
| PW-xyl4 | 369 | Endo-1,4-β-xylanase [Candidatus Atribacteria bacterium] (TFG90307.1) | 73.49 | ✓ |
| PW-xyl5 | 370 | Endo-1,4-β-xylanase [Ignavibacteriae bacterium] (RPI05742.1) | 51.52 | ✓ |
| PW-xyl6 | 372 | Endo-1,4-β-xylanase [Ignavibacteriae bacterium] (RPI05742.1) | 53.68 | |
| PW-xyl7 | 374 | Endo-1,4-β-xylanase [Rufibacter sp. R-22-1c-1] (WP_123126834.1) | 63.71 | ✓ |
| PW-xyl8 | 374 | Endo-1,4-β-xylanase [Paludibacter jiangxiensis] (WP_068704526.1) | 81.01 | ✓ |
| PW-xyl9 | 377 | Endo-1,4-β-xylanase [Bacteroidales bacterium] (NLJ21332.1) | 89.30 | ✓ |
| PW-xyl10 | 383 | Endo-1,4-β-xylanase [Alkalilimnicola ehrlichii] (WP_116303324.1) | 56.84 | ✓ |
| PW-xyl11 | 383 | Endo-1,4-β-xylanase [Runella sp. YX9] (WP_114464289.1) | 69.94 | ✓ |
| PW-xyl12 | 383 | Endo-1,4-β-xylanase [Runella sp. YX9] (WP_114464289.1) | 70.45 | ✓ |
| PW-xyl13 | 390 | 1,4-β-xylanase [Flavobacteriia bacterium] (RUA15923.1) | 58.79 | ✓ |
| PW-xyl14 | 391 | Endo-1,4-β-xylanase [Confluentibacter lentus] (WP_100611294.1) | 71.90 | ✓ |
| PW-xyl15 | 396 | TPA: 1,4-β-xylanase [Planctomycetes bacterium] (HAK94543.1) | 77.31 | ✓ |
| PW-xyl16 | 403 | 1,4-β-xylanase [Butyrivibrio sp. NC2007] (WP_022770809.1) | 52.21 | |
| PW-xyl17 | 413 | TPA: 1,4-β-xylanase [Cyanobacteria bacterium UBA11371] (HAZ45611.1) | 48.50 | ✓ |
| PW-xyl18 | 415 | Endo-1,4-β-xylanase, GH35 family [Bacteroidales bacterium 6E] (GAP68157.1) | 58.29 | |
| PW-xyl19 | 420 | Endo-1,4-β-xylanase [Planctomycetaceae bacterium] (RPJ36220.1) | 74.31 | |
| PW-xyl20 | 422 | 1,4-β-xylanase [Microbispora sp. GKU 823] (WP_079313614.1) | 63.19 | ✓ |
| PW-xyl21 | 424 | Endo-1,4-β-xylanase [Bacteroidales bacterium 6E] (GAP68157.1) | 64.34 | |
| PW-xyl22 | 425 | Endo-1,4-β-xylanase [Bacteroidales bacterium 6E] (GAP68157.1) | 64.92 | |
| PW-xyl23 | 426 | Endo-1,4-β-xylanase [Bacteroidales bacterium 6E] (GAP68157.1) | 60.21 | |
| PW-xyl24 | 451 | Endo-1,4-β-xylanase Z [Tannerella forsythia] (SCQ20945.1) | 34.57 | |
| PW-xyl25 | 452 | 1,4-β-xylanase [Thermogutta terrifontis] (WP_095416323.1) | 55.15 | |
| PW-xyl26 | 460 | 1,4-β-xylanase [Planctomycetes bacterium B3_Pla] (TKJ36372.1) | 60.67 | |
| PW-xyl27 | 475 | β-1,4-xylanase [Mesotoga infera] (KUK68222.1) | 76.09 | |
| PW-xyl28 | 476 | Endo-1,4-β-xylanase [Cyanothece sp. PCC 7822] (WP_013324907.1) | 27.16 | |
| PW-xyl29 | 492 | 1,4-β-xylanase [Aphanothece sacrum] (WP_124970705.1) | 27.23 | |
| PW-xyl30 | 495 | 1,4-β-xylanase [Chthonomonas calidirosea T49] (CCW34445.1) | 43.40 | |
| PW-xyl31 | 496 | Endo-1,4-β-xylanase [Treponema azotonutricium] (WP_015711732.1) | 69.70 | |
| PW-xyl32 | 502 | 1,4-β-xylanase [Bacteroidetes bacterium GWE2_42_39] (OFY01282.1) | 63.91 | |
| PW-xyl33 | 583 | Endo-1,4-β-xylanase [Geitlerinema sp. PCC 9228] (WP_084639516.1) | 50.00 | |
| PW-xyl34 | 778 | Endo-1,4-β-xylanase [Marinilabilia salmonicolor] (WP_010663012.1) | 93.00 | |
| PW-xyl35 | 647 | Endo-1,4-β-xylanase [Hymenobacter gummosus] (WP_126694067.1) | 44.70 | |
| PW-xyl36 | 711 | Endo-1,4-β-xylanase, GH35 family [Bacteroidales bacterium 6E] (GAP68157.1) | 69.05 | |
| PW-xyl37 | 723 | Endo-1,4-β-xylanase B [uncultured Bacteroides sp.] (SCJ93802.1) | 71.01 | ✓ |
| PW-xyl38 | 724 | Endo-1,4-β-xylanase [Ignavibacteriae bacterium] (RPI05742.1) | 50.14 | ✓ |
| PW-xyl39 | 746 | Endo-1,4-β-xylanase [Moorea producens] (WP_083305227.1) | 51.95 | ✓ |
| PW-xyl40 | 746 | TPA: 1,4-β-xylanase [Cyanobacteria bacterium] (UBA11371] (HAZ45611.1) | 57.59 |
aAA is the length of the amino acid;
bIf the last column is labeled
✓, the expressed xylanase gene has xylanase activity