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. 2021 Jan 19;4:84. doi: 10.1038/s42003-020-01600-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Box sizes along x-axis (x-dim) and y-axis (y-dim) distribution over the last 750 ns of three systems over 1.75 µs. b Anisotropic membrane curvature in xz or yz plane, plotted against x-dim or y-dim. The star symbol marks the average values of the last 750 ns trajectory (6250 frames). c Flattening angle of the Piezo1 arms plotted against x-dim. The angle β is defined by the angle between each beam, and the Piezo internal axis defined by the COM of cap and CTD region, illustrated on the right. The mean value of arm2 and arm3 from the last 750 ns are plotted here. The star symbol marks the average values. d V2476 centroid distances are plotted against x-dim. The pore helix of subunit 1 (pore1) is in contact with arm2 and the pore helix of subunit 2 (pore2) with arm3. The mean value of pore1 and pore2 from the last 750 ns are plotted here. The star symbol marks the average values. In c and d, the corresponding values in the cryo-EM structure are indicated by purple dashed lines.