Figure 5.

Under polyclonal stimuli the T cells from persons with interstitial lung abnormalities (ILA) secrete a specific pro-inflammatory profile. Enriched T cells were cultured 48 hours with polyclonal stimuli either with monoclonal antibodies anti-CD3 and CD28 (both at 1 µg/mL) or phorbol myristate acetate (PMA) 50 nM/ionomycin (IO) 2 ng/µL; culture medium (Media) and isotype control (I.C) to monoclonal antibodies were used as negative controls. Culture supernatant was used to evaluate several molecules by Multiplex. We observed diverse behaviour in the profile of delivered pro-inflammatory cytokines; a first cytokine group was spontaneously release (stimuli-independent), a second group showed increased level and a third group showed decreased level in response to polyclonal stimuli (panel A, B and C, respectively). Graphs show individual value mean±SD (n=5 per group). *p<0.05 **p<0.01 ***p<0.001 compared old versus old+ILA, statistical analysis was performed with one-way analysis of variance tests and adjusted by the Holm-Sidak method for multiple comparisons. IFN-γ, interferon gamma; IL, interleukin.